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Discussion Worldwide, tobacco smoking leads to more than 7 million deaths per year and more than 16 million reports for smoking-related diseases in the United States of America (USA) (CDC, 2020c)

Cavendish tobacco is not a specific variety but rather a processing method that involves steaming and flavoring, often resulting in a smooth, aromatic smoke with enhanced sweetness

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Subcellular fractionation Nuclear, mitochondrial and cytosolic fractions were separated using the Mitochondria Isolation Kit for Cultured Cells (89874, ThermoFisher Scientific) according to the manufacturers specifications